General
Research

 

Research Fellow (Bioimage Bioinformatician)

We are looking for a talented and highly motivated postdoctoral fellow to join the Microbial Biofilm group under the supervision of Prof. Yehuda Cohen. The group operates a high-density imaging acquisition platform with cutting edge technologies dedicated to the study of heterogeneous microbial biofilm communities and quantification of molecular microbial ecology, using confocal microscope, matrix screener and dissecting laser microscope.

Contract is initially awarded for 2 years, and may be extended.

Responsibilities:

  • Establish image analysis workflow
  • Interface image analysis with microscope for automation (Leica CAM interface)
  • Feature extraction from multidimensional confocal microscopy data
  • Sensitivity analysis and establishment of key features for data mining
  • Data mining using advanced statistical tools for automation of microscopy
  • Data visualisation of high dimensional data sets

Educational Requirements:

Doctoral degree in Computer Science, Physics, Mathematics, Engineering.

 Required Experience:

  • PhD required in one of the following: bioinformatics, computer science (preferentially with some complementary background in molecular biology/microbiology), statistics (with significant computational expertise)
  • Highly analytical, critical thinker and highly meticulous in work
  • Experience in working with image analysis preferentially on microscopy data, Filtering, Segmentation, Registration and Motion Tracking
  • Strong knowledge of C#, Java, Python, Matlab or other Unix/Linux operating system
  • With Data mining skills: Multiparameter regression, Non-linear regression, Machine learning (Supervised learning, unsupervised learning), Statistics language (R, SAS, SPSS, Matlab (statistics package) or similar)
  • Excellent verbal and written communication skills in English

Interested applicants should submit curriculum vitae, a detailed letter of interest, and the names of three potential referees to SCELSE-HR@ntu.edu.sg. Please indicate 'Research Fellow (Bioimage Bioinformatician)' in the subject line.


Research Fellow

Description:

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking a highly motivated individual to join our research team as a Research Fellow. This post will undertake research investigating polymicrobial human infections and aims to evaluate pathogenic mechanisms used by bacteria within the host. Our studies use a variety of approaches, ranging from in vitro biofilm studies to in vivo murine models, and employ technologies including super-resolution imaging, metagenomics, transcriptomics, and metabolomics.

Requirements:

The successful candidate should hold a recent PhD in biology, microbiology, molecular biology, or related field. Specific expertise in cellular or animal models of infection, analysis and interpretation of omics data, and/or analysis of innate responses to infection are preferred. The successful candidate will also demonstrate strong interpersonal and communication skills, both oral and written.

Interested candidates should submit a current CV including potential references and submit a cover letter with the subject heading “Application for Research Fellow” to Nanyang Asst. Prof. Kimberly Kline at KKline@ntu.edu.sg


Research Fellow (Imaging)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking a Research Fellow to both oversee our Advanced Biofilm Imaging Facility and to carry out research in advanced optical microscopy development.

Description:

  • Provide expertise to develop novel technologies for advanced microbiome research and application

  • Develop image acquisition and image processing capacities into image bioinformatics

  • Select, apply and optimise molecular technologies for optical observations of biofilm/microbiome studies

  • Develop and implement novel/advanced imaging modes/microscopes in support of needs arising from microbiologists and other researchers in collaboration with colleagues at NTU and NUS

  • Manage the operation and technical development of SCELSE’s advanced imaging platform presently including: epifluorescence microscopes, confocal microscopes, superresolution microscope, LightSheet microscope and micro-dissecting scope

  • In collaboration with manufacturers maintain existing microscopes to ensure that they are in good working order

  • Set up policy guidelines and train end users i.e. PIs, Research Fellows and PhD students on proper handling of the microscopes

  • Provide technical support and method development during the upgrade of existing imaging platforms to meet experimental demands

Requirements: 

  • A recognized PhD degree in Science/Physics/Biophysics or related field

  • Extensive experience in developing/building and optimising optical microscopes

  • Experience in advanced light microscopy techniques (e.g. confocal, super-resolution, light sheet, etc.)

  • Working knowledge in programming (e.g. C, Matlab, Python, LabView, etc.) applied to data acquisition and image processing (e.g. Comstat, Imaris, etc.) is necessary

  • Experience in managing a central imaging centre for multiple users

  • Independent and self-motivated

  • Possess strong written and verbal communication skills with good interpersonal skills

Interested candidates should submit a current CV including potential references and must submit a cover letter with the subject heading “Application for Research Fellow (Imaging)” to SCELSE-HR@ntu.edu.sg.

 


Research Assistant (Next-Gen Sequencing) 

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking to appoint a talented individual to join our team at SCELSE as a Research Assistant (Next-Gen Sequencing).

Key Responsibilities:

  • Quality control of incoming samples
  • DNA, RNA and amplicon library construction for various next-generation sequencing platforms
  • Operation of next-generation sequencing instruments and associated equipment
  • Troubleshooting and correction of suboptimal results under the guidance of scientific staff
  • Assistance in the improvement of existing protocols for sample preparation and library construction
  • Quantification of nucleic acids by real time PCR
  • DNA and RNA isolation from various sources
  • General laboratory and instrument maintenance

Requirements:

  • BSc degree in Biological Sciences or related field and/or combination of education and experience
  • Strong background in Genomics and Molecular Biology required
  • Solid knowledge and hands-on experience with molecular biological methods such as handling nucleic acids (DNA and RNA), enzymatic manipulation of nucleic acids, PCR and qPCR required
  • Hands-on experience with library preparation as well as the operation of next-generation sequencing platforms required
  • Hands-on experience with liquid handling platforms preferred
  • Desire and ability to learn and adapt to rapidly changing protocols and technologies
  • Ability to work independently as well as in a team setting
  • Excellent organisational, documentation and communication skills

Interested candidates should submit a current CV including potential references and submit a cover letter with the subject heading “Application for Research Assistant (Next-Gen Sequencing)” to SCELSE-HR@ntu.edu.sg


Research Assistant in Bioinformatics 

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We seek to recruit a Research Assistant to work as part of a team providing bioinformatics services to research teams within the Centre. The position is embedded within the Integrative Analysis Unit, which hosts a team of 5-7 full-time bioinformatics scientists from predoctoral to postdoctoral level.

 

This position would suit a recent graduate in bioinformatics, computational science or related fields, who seeks to develop their career in scientific computing in the context of biological, biomedical or environmental sciences. The majority of the work involves processing next generation (NGS) sequencing data, including shotgun, amplicon sequencing and long read data (Nanopore, PacBio). Essential for this position is experience, interest and aptitude in the analysis and interpretation of real, complex data, including the willingness and ability to undertake data wrangling, and the ability to maintain deadlines involving multiple, simultaneous input data streams. In addition, more specific technical knowledge that would be highly regarded includes: development of web based apps and experience with reproducible research practices or knowledge of graph databases such as Neo4j.

 

We are looking for a proactive, motivated young data scientist, who is unfazed by technical challenges, and who can work effectively and effortlessly within a highly interactive team environment. Accordingly, excellent oral and written communication skills are mandatory, as are the soft skills required to be a committed team player. A detailed knowledge of Unix is mandatory, as is some experience running jobs on high performance computing systems. Coding skills in R, Python, Matlab or comparable data focused languages will be highly regarded. Dedicated high performance computing facilities are available at SCELSE, and further capability is available via the National Supercomputing Centre (NSCC).

  

Please email Rohan Williams (Head, SCELSE Integrative Analysis Unit) at lsirbhw@nus.edu.sg for further information and expression-of-interest.


Research Fellow (Environmental Bacterial Genomics)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University and National University of Singapore. Hosted by the NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

SCELSE seeks to recruit a Post-doctoral Fellow in bioinformatics to work on the comparative genomics of anaerobic ammonia-oxidising bacteria (AnAOB).

We are seeking a highly motivated individual who has expertise and interests in comparative genome analysis to work on anaerobic ammonia-oxidising bacteria (AnAOB) within the context of an interdisciplinary research programme on the microbial ecology of wastewater treatment. The foundation to the project is a cohort of draft genomes, assembled from enrichment reactor metagenome analysis, and complemented with long read (Nanopore) data. Data collection is ongoing, and in the future will include HiC data from these samples.

The applicant must hold a PhD in bioinformatics or computational biology, with demonstrable knowledge and experience in comparative genome analysis and large scale annotation procedures. Skills and experience in any of the following specific areas will be highly regarded: metagenome assembly and hybrid (short-read/long read) assembly procedures, protein structure prediction or an understanding of microbial physiology or enzymology. The successful candidate must hold high level coding skills relevant to data analysis (e.g. preferably R but Python, Matlab or comparable language), as well as a commitment to reproducible research practices.

Excellent written and oral communication skills are mandatory, as are the soft skills required to operate effectively and effortlessly within a highly interactive team environment. Development of open access software outputs is encouraged.

The position is available for an initial period of 1 year, with prospect of renewal.

Interested candidates should submit a current CV, including potential references and a cover letter with the subject heading “Application for Research Fellow (Environmental Bacterial Genomics)” to Dr Rohan Williams (Head of Integrative Analysis Unit, SCELSE) lsirbhw@nus.edu.sg for expression-of-interest. 


Research Assistant (Coral)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a Research Centre of Excellence in Singapore fully funded by the Singapore government through the Ministry of Education and the National Research Foundation, with a mandate to develop in depth knowledge of how microbial communities function.  SCELSE is looking for a Research Assistant to work on a project funded by the National Research Foundation to do ecological surveys to assess the frequency and extent of sediment impact and coral-macroalgal interactions.  In addition, controlled experiments will be performed at St. John’s Island National Marine Laboratory (SJINML) to investigate sediment impact on four selected species of coral.  Following the controlled experiments testing sediment stress and coral-macroalgal interactions at the SJINML and in the field respectively, we will measure community changes in the symbiotic algae and the host microbiome, and compare gene expression profiles of the coral holobiont in response to each treatment.  Finally, to profile the coral holobiont subjected to sediment stress and coral-macroalgal contact in the field, we will take a metabarcoding approach to identify the DNA sequences present within homogenised tissue samples obtained from the tagged colonies. 

Requirements: 

The candidate should have a recognised bachelor’s degree with strengths in molecular biology, meta-genomics and/or meta-transcriptomics or coral ecology.  Excellent writing and communication skills are essential as is the ability to work in a team-based environment.  A dive certificate is also advantageous.

Interested candidates should submit a current CV including potential references and cover letter with the subject heading “Application for Research Assistant (Coral)” to SCELSE-HR@ntu.edu.sg 

 


Research Assistant (High-Throughput Sequencing Lab)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by the NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We seek to appoint a talented individual to join our team at SCELSE as a Research Assistant (High-Throughput Sequencing Laboratory).

Key Responsibilities:

  •           Quality control of incoming DNA and RNA samples

  •           Manual and automated library construction for various sequencing platforms

  •           Quality control of finished libraries by real time PCR

  •           Setting-up of sequencing runs and operation of the latest state-of-the-art sequencing instruments

  •           Tracking of samples, reagents and sequencing data through a LIMS system

  •           Troubleshooting and correction of suboptimal results under the guidance of scientific staff

  •           General laboratory and instrument maintenance

Requirements:

  •           Bachelor’s degree in Biological Sciences or related field and/or combination of education and experience

  •           Strong background in Genomics and Molecular Biology required

  •           Solid knowledge and hands-on experience with molecular biological methods such as handling nucleic acids (DNA and RNA), enzymatic manipulation of nucleic acids, PCR and qPCR required

  •           Hands-on experience with library preparation and/or operation of next-generation sequencing platforms would be an advantage

  •           Hands-on experience with liquid handling platforms would be highly valued

  •           Desire and ability to learn and adapt to rapidly changing protocols and technologies

  •           A high level of attention to detail is absolutely mandatory

  •           Ability to work with large number of samples

  •           Ability to work independently as well as in a team setting

  •           Excellent organisational, documentation and communication skills

Interested candidates should submit a current CV including potential references and must submit a cover letter with the subject heading “Application for Research Assistant (High-Throughput Sequencing Lab)” to SCELSE-HR@ntu.edu.sg


Research Fellow (Biology)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

The Microbial Biofilm group is currently seeking a new Research Fellow to take part in an ongoing research project in the field of biofilm bioengineering. The candidate will be required to perform in vitro bacterial biofilm work, extensively work with confocal microscopy, processing and analysing images, and develop standard operating procedures. In addition, the candidate will also be in charge of routine lab maintenance and most of the administrative tasks.

Requirements:

  • PhD in biology or related degree.
  • Previous full-time experience working in a microbiology laboratory.
  • Strong oral and written communication skills.
  • Demonstrated ability to work both independently and in a team.
  • Attention to detail, ability to organise time efficiently, and a desire to follow projects to completion.
  • Experience in confocal microscopy is essential.
  • Experience in mechanical engineering would be a significant added value.
  • Experience in tasks such as ordering items, and interacting with sales representatives and administrative staff is desired.

Interested applicants for the above position should send a cover letter describing their research experience, interests, curriculum vitae to SCELSE-HR@ntu.edu.sg. Please indicate ‘Research Fellow (Biology)’ in the subject line.


Research Assistant (Cell Culture and Microbiology)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

The microbial biofilms group is currently looking for a motivated and proactive Research Assistant to help undertake experiments investigating the interactions of mixed species biofilms with tissue culture cells. The team is involved in multi-disciplinary research across various fields and works very closely with the clinical research team and also external partners possessing diverse research expertise. 

The successful candidate for this position should be experienced in handling mammalian cell cultures and also possess skills for performing microbiological research. Experience with microbial-mammalian co-cultures would be an asset for this position. The candidate would work towards establishment of cell biology strategies to test the role of microbes and microbe related factors on cellular physiology. This is with an aim to decipher signatures unique to various phenotypes of interest to SCELSE and would involve working with an external industrial partner. The candidate would report to the microbiology project leader and is expected to be a team player with an active interest in carrying out inter-disciplinary research, particularly at the interface of Microbiology and Cell Biology.

Essential Skills:

  • Strong academic credentials - BSc in Biological Sciences
  • Ability to quickly learn and apply newly acquired knowledge
  • Demonstrated experience in handling and maintaining tissue culture cells, and the ability to grow and maintain bacteria and/or fungi
  • Experience in practical laboratory techniques
  • Active interest in inter-disciplinary research work
  • Good organisational skills and adaptability to regularly changing priorities and workloads
  • Effective communication skills and experience in collaborative research projects and inter-group communication

Interested applicants should submit curriculum vitae, a detailed letter of interest, and the names of three potential referees to SCELSE-HR@ntu.edu.sg. Please indicate ‘Research Assistant (Cell Culture and Microbiology)’ in the subject line.


Research Assistant (Analytical Chemistry/Life Sciences)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking a talented and motivated individual to join us as a Research Assistant.

Responsibilities:

  •           Provide research team with biochemistry and analytical support including sample preparation, analyses and data collection.
    •           Assist in general maintenance of analytical instruments, for example, preparing solutions and reagents, replacing consumables on existing equipment, implementing supply and equipment ordering procedures, setting up and maintaining equipment, and so forth.
    •           Develop and verify existing or new analytical methods required by researchers.
    •           Maintain records of research and lab operations; such as documenting experimental research procedures and risk assessment.
    •           Perform routine molecular biology lab duties such as DNA, RNA and protein extractions.

Requirements:

Essential

  •           Polytechnic diploma and/or degree (e.g. BSc) in chemistry, life sciences or related field.
  •           Previous full-time experience working in a research laboratory.
  •           Demonstrated ability to work both independently and in a team.
  •           Attention to detail, ability to organise time efficiently, and a desire to follow projects to completion.
  •           Strong oral and written communication skills.

Desirable

  •           Experience in operating gas chromatography (GC), high performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy.
  •           Experience in purification of complex biopolymers using molecular size based techniques, such as size exclusion chromatography (SEC), gel electrophoresis, etc.
  •           Experience in fluorescence and imaging technique including scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM).
  •           Experience carrying out functions such as ordering, and interacting with sales representatives and administrative staff are a plus.

Interested applicants should submit a current CV including potential references, and a cover letter with the subject heading “Application for Research Assistant (Analytical Chemistry/Life Sciences)” to SCELSE-HR@ntu.edu.sg.

 


 

Research Assistant/Associate (MSRDP)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation (NRF), Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

 

We are looking for a Research Associate to work on a project funded by NRF to address the fate and effects of human and fish pathogens in coastal waters.  Controlled experiments will be performed at St. John’s Island National Marine Laboratory (SJINML) using flumes. A total of four flume-based experiments, each lasting about 1.5 months, will be performed. Sample and data processing will be performed at SCELSE, NTU.

 

Responsibilities

·        Provide researchers with support including experiment planning, sample preparation, analyses and data collection.

·        Assist in the general maintenance of flumes and other equipment, for example, cleaning, replacing consumables, implementing supply and equipment ordering procedures, setting up equipment and so forth.

·        Maintain records of research and lab operations; this includes documenting experimental research procedures and risk assessments, and assisting with laboratory database management.

·        Assist researchers in on-site sample collection, including preparation of consumables, transportation and sample processing.

·        Perform routine molecular biology lab duties.

Requirements:

·        Polytechnic diploma and/or Bachelor’s/Master’s degree in chemistry, life sciences or related field.

·        Previous full-time experience working in a research laboratory.

·        Experience in performing RNA and DNA extractions, qPCR, gel electrophoresis.

·        Willing to travel approximately 3-4 days per week (may include weekends/PH) to SJINML during the flume experiments. Previous experience with marine systems or reactors is a plus.

·        Strong oral and written communication skills.

·        Experience carrying out functions such as ordering, interacting with sales representatives and administrative staff is a plus.

·        Demonstrated ability to work both independently and in a team.

·        Attention to detail, ability to organise time efficiently and a desire to follow projects to completion.

·        Valid Class 3 (manual) driving license for at least 2 years.

Interested candidates should submit a current CV including potential references and must submit a cover letter with the subject heading “Application for Research Assistant/Associate (MSRDP)” to SCELSE-HR@ntu.edu.sg.